Biodegradation of DDT by Co-cultures of Pleurotus eryngii and Pseudomonas aeruginosa

  • Diana Maulianawati Department of Aquaculture, Faculty of Fisheries and Marine Science, Borneo Tarakan University, Tarakan
  • Adi Setyo Purnomo Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Surabaya
  • Ichiro Kamei Department of Forest and Environmental Science, Faculty of Agriculture, University of Miyazaki, Miyazaki

Abstract

Dichloro-diphenyl-trichloroethane (DDT) is a synthetic insecticide that widely used around the world, which has a negative effect on human health and the environment. The objective of this research was to investigate the ability of bacterium Pseudomonas aeruginosa in co-culturing with white-rot fungus Pleurotus eryngii to degrade DDT. The various volume of P. aeruginosa (1 ml ≈ 1.5 x 109 CFU) were added into 10 ml of P. eryngii culture for a 7-days of incubation. Approximately 82% of degradation of DDT were obtained from co-cultures with the adjunct of 10 ml of P. aeruginosa during the 7-day incubation period, which had the best ratio of optimization of 0.57. The confrontational assay showed that P. aeruginosa gave no effect on the growth of P. eryngii (0.39 cm/day). DDD (1,1-dichloro-2,2-bis(4-chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene) were detected as metabolic products from the DDT degradation by co-cultures bacterium P. aeruginosa and fungus P. eryngii. This study indicated that bacterium P. aeruginosa can be used to enhance DDT degradation by whire-rot fungus P. eryngii.

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Published
2021-08-25
How to Cite
Maulianawati, D., Purnomo, A. S., & Kamei, I. (2021). Biodegradation of DDT by Co-cultures of Pleurotus eryngii and Pseudomonas aeruginosa. HAYATI Journal of Biosciences, 28(3), 240. https://doi.org/10.4308/hjb.28.3.240
Section
Articles