Quality Improvement of White Pepper with Processing through Optimizing the Ratio of Starter Culture from Acetobacter sp., Bacillus subtilis, and Bacillus cereus

  • Kirana Sanggrami Sasmitaloka Indonesian Center for Agricultural Postharvest Research and Development, Bogor, Indonesia
  • Hernani Hernani Indonesian Center for Agricultural Postharvest Research and Development, Bogor, Indonesia
DOI: https://doi.org/10.4308/hjb.29.1.87-96

Abstract

Fermentation process using known microbial species can be exploited for the processing of white pepper. It is expected to generate quality of white pepper in a short period soaking time. This research aimed to study characteristics of white pepper through a fermentation process by addition of combination isolates of Acetobacter sp., B. subtilis, and B. cereus. After threshing, 2 kg of fresh pepper berries was soaked in water mixed with starter culture. The experiment used a completely randomized design, two replications. The treatment consisted of: A) starter culture ratio of Acetobacter sp.: B. subtillis: B. cereus (A1 = 0:0:0; A2 = 1:1:1; A3 = 2:1:1; A4 = 1:2:1; and A5 = 1:1:2) and soaking time (B1 = 5 and B2 = 7 days). Fermented pepper was decorticated, washed, and dried. The best treatment was fermented for 7 days with the combination isolates of Acetobacter sp., B. subtilis, and B. cereus with ratio 2:1:1. This condition produced white pepper in fulfilling in requirement of SNI standards with piperine and essential oil contents and TPC of 5.95%, 2.95% and 1.1 x 102 CFU/g, respectively. This process is expected to generate high quality of white pepper in a short soaking time.

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Published
2021-12-09
How to Cite
SasmitalokaK. S., & HernaniH. (2021). Quality Improvement of White Pepper with Processing through Optimizing the Ratio of Starter Culture from Acetobacter sp., Bacillus subtilis, and Bacillus cereus. HAYATI Journal of Biosciences, 29(1), 87-96. https://doi.org/10.4308/hjb.29.1.87-96
Issue
Vol. 29 No. 1 (2022): January 2022
Section
Articles

Copyright (c) 2022 Kirana Sanggrami Sasmitaloka, Hernani Hernani

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