Eksplorasi dan Karakterisasi Bakteri Agens Hayati dari Imperata cylindrica untuk pengendalian Rigidoporus microporus

  • Tatit Sastrini Balai Pengkajian Teknologi Pertanian, Sumatera Barat 27366
  • Mochamad Yadi Nurjayadi Balai Proteksi Tanaman Perkebunan Pontianak, Kalimantan Barat 78241
  • abdul munif Institut Pertanian Bogor

Abstract

Exploration and Characterization of Biological Agents Bacteria from Imperata cylindrica for Controlling Rigidoporus microporus

White root disease caused by Rigidoporus microporus is an important disease in rubber plantation. This pathogen causes damage to roots, thus adversely affect plant growth and productivity of rubber plantation. Therefore controling of this pathogen is important to do. The objective of this research was to obtain biological agents bacteria from the various parts of alang-alang (Imperata cylindrica) which have inhibitory activity against the growth of R. microporus The research was conducted through exploration of biological agents from the various parts of alang-alang, antibiosis activity test, and characterization of potential isolates. Based on antibiosis activity test, seven isolates (Rph 7, Rph 8, Rph 15 Rph 17, 15, E47, and isolate 47) showed antibiosis activity against R. microporus. Suppression activity of R. microporus growth by seventh of potential isolates ranged between 26.7-58.9%. The highest inhibition was demonstrated by isolate 47 suspected leaf endophytic bacteria. Seventh of potential isolates are Gram-positive bacterial that can form spores. The biological agen’s bacterial dominated by isolates from the reeds rhizosphere. This result indicates that rhizosphere is a potentialy source in the exploration of biological agents. In addition, R. microporus is a soil-borne pathogen which colonizes the roots therefore exploration from rhizosphere samples allows obtaining potential biological agents.

Downloads

Download data is not yet available.

Author Biography

abdul munif, Institut Pertanian Bogor
Plant Protection

References

Aktar W, Sengupta D, Chowdhury A. 2009. Impact of pesticides use in agriculture: their benefits and hazards. Interdisciplinary Toxicology. 2(1):1–12. DOI: https://doi.org/10.2478/v10102-009-0001-7.

Amaria W, Taufiq E, Harni R. 2013. Seleksi dan identifikasi jamur antagonis sebagai agens hayati jamur akar putih (Rigidoporus microporus) pada tanaman karet. Buletin RISTRI. 4(1):55–64. DOI: https://doi.org/10.21082/jtidp.v1n2.2014.p79-86.

Baker KF, Cook RJ. 1974. Biological Control of Plant Pathogens. San Francisco (US): WH Freeman.

Berebdsen RL, Pieterse CMJ, Bakker PAHM. 2012. The rhizosphere microbiome and plant health. Trends Plant Sci. 17(8):478–486. DOI: https://doi.org/10.1016/j.tplants.2012.04.001.

Bivi MR, Farhana MS, Khairulmazmi, Indist A. 2010. Control of Ganoderma boninense: A causal agent of basal stem rot disease in oil palm with endophyte bacteria In Vitro. Int J Agric Biol. 12:833–839.

Cao LX, Qiu ZQ, You JL, Tan HM, Zhou S. 2004. Isolation and characterization of endophytic Streptomyces antagonists of Fusarium wilt pathogen from surface sterilized banana roots. FEMS Microbiol Lett. 247:147–152. DOI: https://doi.org/10.1016/j.femsle.2005.05.006.

D’Antonio C, Meyerson LA. 2002. Exotic plant species as problems and solutions in ecological restoration: a synthesis. Restoration Ecol. 10(4):703–713. DOI: https://doi.org/10.1046/j.1526-100X.2002.01051.x.

Emmert EAB, Handelsman J. 1999. Biological of plant disease: a (Gram-) positive perspective. FEMS Microbiology Letters. 171:1–9. DOI: https://doi.org/10.1111/j.1574-6968.1999.tb13405.x.

Hagan DL, Jose S, Bohn K, Escobedo F. 2013. Cogongrass (Imperata cylindrica) invasion and eradication: Implications for soil nutrient dynamics in a longleaf pine sandhill ecosystem. Invasive Plant Sci and Manage. 6(3):433–443. DOI: https://doi.org/10.1614/IPSM-D-12-00089.1.

Hallmann J, Hallmann AQ, Mahaffee WF, Kloepper JW. 1997. Bacterial endophytes in agricultural crops. Can J Microbiol. 43:895–914. DOI: https://doi.org/10.1139/m97-131.

Hastuti RD, Lestari Y, Suwarso A, Saraswati R. 2012. Endophytic Streptomyces spp. as biocontrol agents of rice bacterial leaf blight pathogen (Xanthomonas oryzae pv. oryzae). Hayati. 19(4):155–162. DOI: https://doi.org/10.4308/hjb.19.4.155.

Jayasuriya KE, Thennakoon BI. 2007. Biological control of Rigidoporus microporus, the cause of white root disease in rubber. Cey J Sci (Bio Sci). 36(1):9–16.

Jose S, Cox J, Miller DL, Shilling DG, Merritt S. 2002. Alien plant invasions: the story of cogongrass in southeastern forests. J Forestry. 100 (1):41–44.

Khaerani A, Rahman A. 2012. Penggunaan bakteri kitinolitik sebagai agens biokontrol penyakit busuk batang oleh rhizoctonia solani pada tanaman kedelai. J Fitopatol Indones. 8(2):37–43. DOI: https://doi.org/10.14692/jfi.8.2.37.

Kusumawati DI, Widawati S, Lisdiyanti P, Sudiana IM. 2017. Isolation and screening for IAA production, nitrogen fixation, P-solubilization and cellulolytic activity of plant growth-promoting rhizobacteria from Imperata cylindrica Grasslands. Di dalam: Prosiding The Project for Producing Biomass Energy and Material through Revegetation of Alang-alang (Imperata cylindrica) Fields; 2016 Nov 14; Bogor (ID): Indonesia Institute of Sciences. Hlm 125–133.

Krzyzanowska DM, Iwanicki A, Ossowicki A, Obuschowski M, Jafra S. Genome sequence of Bacillus subtilis MB73/2, a soil isolated inhibiting the growth of plant pathogens Dickeya spp. and Rhizoctonia solani. Genome Announcements. 1(3):e00238-13. DOI: https://doi.org/10.1128/genomeA.00238-13.

Munif A, Wiyono S, Suwarno. 2012. Isolasi bakteri endofit asal padi gogo dan potensinya sebagai agens biokontrol dan pemacu pertumbuhan. J Fitopatol indones. 8(3):57–64. DOI: https://doi.org/10.14692/jfi.8.3.57.

Pal KK, Gardener BM. 2006. Biological Control of Plant Pathogens. The Plant Health Instructor. DOI: https://doi.org/10.1094/PHI-A-2006-1117-02.

Prasetyo J, Aeny TN. 2013. The preventive control of white root rot disease in small holder rubber plantation using botanical, biological and chemical agents. J HPT Tropika. 13(1):69–74.

Schaad NW, Jones JB, Chun W. 2000. Laboratory Guide for Identification of Plant Pathogenic Bacteria. Minnesota (US): APS Press.

Sigee DC. 1993. Bacterial Plant Pathology: Cell and Molecular Aspects. Canbridge (UK): Cambridge University Press. DOI: https://doi.org/10.1017/CBO9780511525476.

Souto GI, Correa OS, Montecchia MS, Kerber NL, Pucheu NL, Bachur M. 2004. Genetic and functional characterization of a Bacillus sp. strain excreting surfactin and antifungal metabolites partially identified as iturin-like compounds. J App Microbiol. 97:1247–1256. DOI: https://doi.org/10.1111/j.1365-2672.2004.02408.x.

Suryanto D, Patonah S, Munir E. 2010. Control of fusarium wilt of chili with chitinolytic bacteria. HAYATI J Biosci. 17(1):5–8. DOI: https://doi.org/10.4308/hjb.17.1.5.

Verma VC, Gond SK, Kumar A, Mishar A, Kharwar RN, Gange AC. 2009. Endophytic actinomycetes from Azadirachta indica A. Juss: Isolation, diversity, and anti-microbial activity. Microb Ecol. 57:749–756. DOI: https://doi.org/10.1007/s00248-008-9450-3.

Published
2019-12-23
How to Cite
SastriniT., NurjayadiM. Y., & munif, abdul. (2019). Eksplorasi dan Karakterisasi Bakteri Agens Hayati dari Imperata cylindrica untuk pengendalian Rigidoporus microporus. Jurnal Fitopatologi Indonesia, 15(2), 69-76. https://doi.org/10.14692/jfi.15.2.69-76
Section
Articles