Aklimatisasi dan Respon Pertumbuhan Mutan Leucaena Leucocephala Varietas Tarramba Teradaptasi Asam

Acclimatization and Growth Respond of The Acid-Adapted Mutant of Leucaena leucocephala cv. Tarramba

  • Muhklisani IPB University
  • P D M H Karti IPB University
  • I Prihantoro IPB University
Keywords: acclimatization, IBA hormone, Leucaena leucocephala, tissue culture

Abstract

Acclimatization is the final stage of plant propagation in tissue culture techniques that can determine the success of the nursery process. This study aimed to observe the growth response during the acclimatization stage of the acid-adapted of Leucaena leucocephala Tarramba variety, which developed from tissue culture techniques. The design used in this study was a completely randomized design (CRD) with the cultivation of 11 mutant lines from tissue culture, namely M1-M11 (an acid-adapted mutant from tissue culture addition of 1 ppm IBA) and 2 controls (lamtoro broodstock without gamma irradiation) namely K0 (lamtoro broodstock resulting from tissue culture addition of 0 ppm IBA), K1 (breeding lamtoro from tissue culture addition of 1 ppm IBA). The variables observed were the level of plant viability, plant height, and number of leaves. The results showed that the acclimatization of the plant Leucaena leucocephala to tissue culture production on the M3 and M9 mutant lines gave the best response to plant morphological growth up to 5 WAP (weeks after planting).

Key words:        acclimatization, IBA hormone, Leucaena leucocephala, tissue culture

Downloads

Download data is not yet available.

References

[IAEA] International Atomic Energy Agency. 2010. Mutant Variety and Genetic Stock Database. Austria (AT): IAEA.

Damayanti D, Sudarsono, Mariska, I & Herman M. 2007. Regenerasi pepaya melalui kultur in vitro. Jurnal AgroBiogen. 3(2): 49-54.

Handini AS. 2012. Pengaruh paclobutrazol terhadap pertumbuhan anggrek Dendrobium lasianthera pada tahap aklimatisasi [skripsi]. Bogor (ID): Fakultas Pertanian, Institut Pertanian Bogor.

Manpaki SJ, Karti, PDM & Prihantoro I. 2017. Respon pertumbuhan eksplan tanaman lamtoro (Leucaena leucocephala cv. tarramba) terhadap cekaman kemasaman media dengan level pemberian aluminium melalui kultur jaringan. Jurnal Sain Peternakan Indonesia. 12(1):71-82.

Marga A. 2020. Perakitan tanaman lamtoro (Leucaena leucocephala) terdaptasi tanah masam. [tesis]. Fakultas Peternakan. Bogor (ID) : Institut Pertanian Bogor.

Santoso U & Nursandi F. 2004. Kultur Jaringan Tanaman. Malang (ID) : UMM Pres.

Sarwono, B. 1987. Pengelompokan Hijauan Makanan Ternak. Jakarta (ID): Trubus.

Sigit Y. 2008. Kajian elongasi dan aklimatisasi pada kultu in vitro Aquilaria beccariana van Tiegh. [skripsi]. Bogor (ID) : Fakultas Kehutanan, Institut Pertanian Bogor.

Soedjono S. 2003. Aplikasi mutasi induksi dan variasi somaklonal dalam pemuliaan tanaman. Jurnal Penelitian dan Pengembangan Peetanian 22(2):34-40.

Wattimena GA, Wiendi NM, Ansori N, Purwito A. Efendi D, Khumaida N & Purwoko BS. 2011. Bioteknologi dalam Pemuliaan Tanaman. Bogor (ID): IPB Press.

Yusnita. 2003. Kultur Jaringan: Cara Memperbanyak Tanaman secara Efisien. Jakarta (ID) : Agro Media Pustaka

Yusnita. 2010. Perbanyakan In vitro Tanaman Anggrek. Bandar Lampung (ID) : Universittas Lampung

Zayed MZ, Ahmad FB, Zaki MA, Ho WS & Pang SL. 2014. The reduction of mimosine content in Leucaena leucocephala (petai belalang) leaves using ethylmethanesulphonate (EMS). Archives of Applied Science Research. 6(4):124-128.

Zulastri R, Karti PDMH & Mutia R. 2020. Aklimatisasi pada tanaman lamtoro (Leucaena leucocephala) cv. Tarramba pasca irradiasi sinar gamma dan sifat tumbuh yang berbeda. [skripsi]. Bogor (ID) : IPB University.

Zulkarnain. 2009. Kultur Jaringan Tanaman Solusi Perbanyakan Tanaman Budidaya. Jakarta (ID) : Bumi Aksara

Irwan Z. 2020. Kadar zat nutrien daun kelor (Moringa oleifera) berdasarkan metode pengeringan. Jurnal Kesehatan Manarang. 6 (1): 69 – 77.

Koten BB, Soetrisno RD, Ngadiyono N, & Soewignyo B. 2014. Perubahan nilai nutrient tanaman sorgum (Sorghum bicolor (L.) Moench) varietas local rote sebagai hijauan pakan ruminansia pada berbagai umur panen dan dosis pupuk urea. Pastura. 3 (2) : 55-60.

Kumalasari NR, Wicaksono GP, & Abdullah L. 2017. Plant growth pattern, forage yield, and quality of Indigofera zollingeriana influenced by row spacing. Media Peternakan. 40 (1):14-19.

doi: https://doi.org/10.5398/medpet.2017.40.1.14.

Laka M &Wangge ESA. 2018. Uji kadar protein pada beberapa varietas umbi ubi kayu (Manihot esculenta Crantz) yang dihasilkan di Desa Randotonda, Kecamatan Ende, Kabupaten Ende. AGRICA. 11 (1) : 43-50.

Masganti, Anwar K, & Susanti MA. 2017. Potensi dan pemanfaatan lahan gambut dangkal untuk pertanian. Jurnal Sumberdaya Lahan. 11 (1): 43-52.

Masganti, Wahyunto, Dariah A, Nurhayati, & Yusuf R. 2014. Karakteristik dan potensi pemanfaatan lahan gambut terdegradasi di Provinsi Riau. Jurnal Sumberdaya Lahan. 8 (1): 59-66.

Savitri MV, Sudarwati H, & Hermanto. 2013. Pengaruh umur pemotongan terhadap produktivitas gamal (Gliricidia sepium). Jurnal Ilmu-Ilmu Peternakan. 23 (2): 25-35.

Setiyaningrum E, Kaca IN, & Suwitari NKE. 2018. Pengaruh umur pemotongan terhadap produksi dan kualitas nutrisi tanaman indigofera (Indigofera sp). Gema Agro. 23 (1): 59-62. doi:10.22225/ga.23.1.660.59-62.

Sriagtula R, Karti PDM.H, Abdullah L, Supriyanto, & Astuti DA. 2017. Nutrient changes and in vitro digestibility in generative stage of M10-BMR sorghum mutant lines. Media Peternakan. 40 (2):111-117.

Tantalo S, Liman, & Fathul F. 2019. Efek umur pemangkasan indigofera (Indigofera zollingeriana ) pada musim kemarau terhadap kadar neutral detergent fibre dan acid detergent fibre. Jurnal Ilmiah Peternakan Terpadu. 7(2): 241-246.

Tarigan A, Abdullah L, Ginting SP, & Permana IG. 2010. Produksi dan komposisi nutrisi serta kecernaan in-vitro Indigofera sp. pada interval dan tinggi pemotongan berbeda. Jurnal Ilmu Ternak dan Veteriner. 15 (2): 188-195.

Vinyard JR, Hall JB, Sprinkle JE, & Chibisa GE. 2018. Effects of maturity at harvest on the nutritive value and ruminal digestion of Eragrostis tef (cv. Moxie) when fed to beef cattle. Journal of Animal Science. 96 (8) :3420–3432. doi:10.1093/jas/sky202.

Yanuartono, Purnamaningsih H, Indarjulianto S, Nururrozi A, Raharjo S, & Haribowo N. 2019. Perlakuan biologis dengan memanfaatkan fungi untuk meningkatkan kualitas pakan ternak asal hasil samping pertanian. Jurnal Peternakan Sriwijaya. 8 (2): 18-34.

Zhao CX, He MR, Wang ZL, Wang YF, Lin Q. 2009. Effects of different water availability at post-anthesis stage on grain nutrition and quality in strong-gluten winter wheat. Comptes Rendus Biologies. 332 (8): 759-764. doi: 10.1016/j.crvi.2009.03.003

Published
2021-12-30
How to Cite
Muhklisani, KartiP. D. M., & PrihantoroI. (2021). Aklimatisasi dan Respon Pertumbuhan Mutan Leucaena Leucocephala Varietas Tarramba Teradaptasi Asam: Acclimatization and Growth Respond of The Acid-Adapted Mutant of Leucaena leucocephala cv. Tarramba . Jurnal Ilmu Nutrisi Dan Teknologi Pakan (Nutrition and Feed Technology Journal), 19(3), 66-70. https://doi.org/10.29244/jintp.19.3.66-70