Comparison of Different Herbal Additives on Immune Response and Growth Performance of Broiler Chickens

  • S. Masoud-Moghaddam Department of Biochemistry, Neyshabur Branch, Islamic Azad University
  • J. Mehrzad Department of Biochemistry, Neyshabur Branch, Islamic Azad University
  • A. H. Alizadeh-Ghamsari Animal Science Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO)
  • R. Bahari Kashani Department of Animal Science, Mashhad Branch, Islamic Azad University
  • J. Saeidi Department of Physiology, Neyshabur Branch, Islamic Azad University
Keywords: herbal additives, broiler chicken, growth performance, immune response, virginiamycin


This experiment was conducted to compare the effects of four commercial herbal additives (Noviherb®, Bioessence®, Biostrong®, and Novigrow®), and a commercial antibiotic (virginiamycin) on growth performance, carcass yield, visceral organs weight, thyroid hormones, and humoral immune responses of broiler chickens. Nine hundred day-old Ross 308 male broiler chicks were reared in litter-covered floor cages and distributed into five experimental groups with six replicates of 30 birds. Five dietary treatments tested were diets supplemented with Noviherb®, Bioessence®, Biostrong® (each of them 100g/ton of diet), Novigrow® (1000g/ton of diet), and virginiamycin (100g/ton of diet) as a positive control. Feed intake, body weight, mortality, feed conversion ratio, European broiler index, and feed cost per kilogram of body weight were measured during the experiment. Antibody titers against sheep red blood cells (SRBC), immunoglobulin G, immunoglobulin M, and plasma concentrations of thyroxine (T4) and triiodothyronine (T3) were evaluated at the age of 42 days. Then, four birds per replicate were killed to determine the relative weight of carcass and organs. The GLM procedures of SAS software and Duncan’s multiple range test were applied to analyze data in a completely randomized design with five treatments and six replicates of 30 birds per each. The effects of herbal additives on productive traits feed cost per kilogram of body weight and relative weight of organs were not significant in comparison with antibiotic treatment. Dietary inclusion of Noviherb®, Biostrong®, and Novigrow® significantly decreased feed cost per kilogram of carcass compared with Bioessence® or antibiotic treatments (p<0.05). The titers of IgG in Noviherb® and virginiamycin were significantly higher (p<0.05) than in Biostrong® and Novigrow® fed groups. The plasma concentration of triiodothyronine was significantly decreased (p<0.05) by dietary inclusion of Noviherb® and Novigrow® compared to the antibiotic. In conclusion, all the evaluated herbal additives could act as a substitute for the growth-promoting antibiotic.


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Adedokun, S. A. & O. C. Olojede. 2019. Optimizing gastrointestinal integrity in poultry: the role of nutrients and feed additives. Front. Vet. Sci. 5:348.

Akeel, R. A., Y. Al-Sheikh, A. Mateen, R. Syed, K. Janardhan & V. C. Gupta. 2014. Evaluation of antibacterial activity of crude protein extracts from seeds of six different medical plants against standard bacterial strains. Saudi J. Biol. Sci. 21:147-151.

Alagawany, M. M., M. R. Farag & K. Dhama. 2015a. Nutritional and biological effects of turmeric (Curcuma longa) supplementation on performance, serum biochemical parameters and oxidative status of broiler chicks exposed to endosulfan in the diets. Asian J. Anim. Vet. Adv. 10:86-96.

Alagawany, M. M., M. R. Farag, K. Dhama, M. E. Abd El-Hack, R. Tiwari & G. M. Alam. 2015b. Mechanisms and beneficial applications of resveratrol as feed additive in animal and poultry nutrition: A review. Int. J. Pharmacol. 11:213-221.

Auezova, L., A. Najjar, M. Kfoury, S. Fourmentin & H. Greige-Gerges. 2020. Antibacterial activity of free or encapsulated selected phenylpropanoids against Escherichia coli and Staphylococcus epidermidis. J. Appl. Microbiol. 128:710-720.

Ali, A. H. H. 2014. Productive performance and immune response of broiler chicks as affected by dietary marjoram leaves powder. Egypt. Poult. Sci. J. 34:57-70.

Aly, S. E., B. A. Sabry, M. S. Shaheen & A. S. Hathout. 2016. Assessment of antimycotoxigenic and antioxidant activity of star anise (Illicium verum) in vitro. J. Saudi Soc. Agric. Sci. 15:20-27.

Amiri, N., M. Afsharmanesh, M. Salarmoini, A. Meimandipour, S. A. Hosseini & H. Ebrahimnejad. 2020. Effects of nanoencapsulated cumin essential oil as an alternative to the antibiotic growth promoter in broiler diets. J. Appl. Poult. Res. 29:875-885.

AOAC. 2012. Official Methods of Analysis of AOAC Interna-tional. 19th ed. Assoc. Off. Anal. Chem., Gaithersburg, Maryland.

Aviagen. 2014. Ross 308: Broiler Nutrition Specifications. [12 April 2016].

Bajpai, V. K., K. -H. Baek & S. C. Baek. 2012. Control of Salmonella in foods by using essential oils: A review. Food Res. Int. 45:722-734.

Bassole, I. H. N. & H. R. Juliani. 2012. Essential oils in combination and their antimicrobial properties. Molecules 17:3989-4006.

Boruga, O., C. Jianu, C. Misca, I. Golet, A. T. Gruia & F. G. Horhat. 2014. Thymus vulgaris essential oil: Chemical composition and antimicrobial activity. J. Med. Life. 7:56-60.

Chahal, K. K., K. Dhaiwal, A. Kumar, D. Kataria & N. Singla. 2017. Chemical composition of Trachyspermum ammi L. and its biological properties: A review. J. Pharmacogn. Phytochem. 6:131-140.

Chandra, A. K. & N. De. 2010. Goitrogenic/antithyroidal potential of green tea extract in relation to catechin in rats. Food Chem. Toxicol. 48:2304-2311.

Clavijo-López V. & M. J. Vives-Florez. 2018. The gastrointestinal microbiome and its association with the control of pathogens in broiler chicken production: A review Poult. Sci. 97:1006-1021.

Cook, N. C. & S. Samman. 1996. Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary sources. J. Nutr. Biochem. 7:66-76.

Dhama, K., S. Chakraborty, R. Tiwari, A. K. Verma, M. Saminathan, Y. S. Amarpal-Malik, Z. Nikousefat, M. Javdani & R. U. Khan. 2014. A concept paper on novel technologies boosting production and safeguarding health of humans and animals. Res. Opin. Anim. Vet. Sci. 4:353-370.

Dhama, K., S. K. Latheef, S. Mani, H. A. Samad, K. Karthik, R. Tiwari, R. Khan, M. Alagawany, M. Farag, G. Alam, V. Laudadio & V. Tufarelli. 2015. Multiple beneficial applications and modes of action of herbs in poultry health and production-a review. Int. J. Pharmacol. 11:152-176.

Diao, W. R., Hu, Q. P., Zhang, H, & J. G. Xu. 2014. Chemical composition, antibacterial activity and mechanism of action of essential oil from seeds of fennel (Foeniculum vulgare Mill.). Food Control 35:109-116.

Diaz-Sanchez, S., D. D’Souza, D. Biswas & I. Hanning. 2015. Botanical alternatives to antibiotics for use in organic poultry production. Poult. Sci. 94:1419-1430.

El-Hack, M. E. A., M. Alagawany, A. M. E. Abdel-Moneim, N. G. Mohammed, A. F. Khafaga, M. Bin-Jumah, S. I. Othman, A. A. Allam & S. S. Elnesr. 2020. Cinnamon (Cinnamomum zeylanicum) oil as a potential alternative to antibiotics in poultry. Antibiotics. 9:210.

El-Sheikh, S. M., M. H. Khairy, N. Z. Eleiwa, O. E. Abdalla & A. G. A. El-Monsef. 2018. Effect of sanguinarine phytobiotic, sodium butyrate compared to ampicillin on controlling necrotic enteritis in broiler chickens. Slov. Vet. Res. 55 (Suppl. 20):405-414.

Farimani, M. M., F. Mirzania, A. Sonboli & F. M. Moghaddam. 2017. Chemical composition and antibacterial activity of Dracocephalum kotschyi essential oil obtained by microwave extraction and hydrodistillation. Int. J. Food Prop. 20:306-315.

Fascina, V. B., J. R. Sartori, E. Gonzales, F. Barros De Carvalho, I. M. G. Pereira De Souza, G. V. Polycarpo, A. C. Stradiotti & V. C. Pelicia. 2012. Phytogenic additives and organic acids in broiler chicken diets. R. Bras. Zootec. 41:2189-2197.

Gadde, U.D., S. Oh, H. S. Lillehoj & E. P. Lillehoj. 2018. Antibiotic growth promoters virginiamycin and bacitracin methylene disalicylate alter the chicken intestinal metabolome. Sci. Rep. 8:3592.

Garcia-Dieza, J., J. Alheiroa, A. L. Pintoa, V. Falcob, M. J. Fraquezac & L. Patarata. 2017. Synergistic activity of essential oils from herbs and spices used on meat products against foodborne pathogens. Nat. Prod. Commun. 12:281-286.

Goy, R. C., S. T. B. Morais & O. B. G. Assis. 2016. Evaluation of the antimicrobial activity of chitosan and its quaternized derivative on E. coli and S. aureus growth. Rev. Bras. Farmacogn. 26:122-127.

Gunal, M., G. Yayli, O. Kaya, N. Karahan & O. Sulak. 2006. The effects of antibiotic growth promoter, probiotic or organic acid supplementation on performance, intestinal microflora and tissue of broilers. Int. J. Poult. Sci. 5:149-155.

Hashemipour, H., H. Kermanshahi, A. Golian & T. Veldkamp. 2013. Effect of thymol and carvacrol feed supplementation on performance, antioxidant enzyme activities, fatty acid composition, digestive enzyme activities, and immune response in broiler chickens. Poult. Sci. 92:2059- 2069.

Hassan, W., S. Gul-Rehman, H. Noreen, Z. Shah, I. Mohammadzai & B. Zaman. 2016. Chemical composition, essential oil characterization and antimicrobial activity of Carum copticum. Vitam. Miner. 5:1-5.

Hassanshahian, M., Z. Bayat, S. Saeidi & Y. Shiri. 2014. Antimicrobial activity of Trachyspermum ammi essential oil against human bacterial. Int. J. Adv. Biol. Biomed. Res. 2:18-24.

Hosseini, S. & A. Meimandipour. 2018. Feeding broilers with thyme essential oil loaded in chitosan nano-particles: An efficient strategy for successful delivery. Br. Poult. Sci. 59: 669-678.

Hussein, E. O. S., S. H. Ahmed, A. M. Abudabos, M. R. Aljumaah, M. M. Alkhlulaifi, M. A. Nassan, G. M. Suliman, M. A. E. Naiel & A. A. Swelum. 2020. Effect of antibiotic, phytobiotic and probiotic supplementation on growth, blood indices and intestine health in broiler chicks challenged with Clostridium perfringens. Animals. 10:507.

Kamely, M., M. A. Karimi Torshizi & S. Rahimi. 2015. Incidence of ascites syndrome and related hematological response in short-term feed-restricted broilers raised at low ambient temperature. Poult. Sci. 94:2247-2256.

Karimi, M., F. Foroudi & M. R. Abedini. 2014. Effect of Sangrovit® on performance and morphology of small intestine and immune response of broilers. Biosci. Biotechnol. Res. Asia. 11:855-861.

Khattak, F., A. Ronchi, P. Castelli & N. Sparks. 2014. Effects of natural blend of essential oil on growth performance, blood biochemistry, cecal morphology, and carcass quality of broiler chickens. Poult. Sci. 93:132-137.

Kini, A., C. Fernandes & D. Suryawanshi. 2016. Effect of KiFAY on Performance, Insulin-like Growth Factor-1, and Thyroid Hormones in Broilers. Asian-Australas. J. Anim. Sci. 29:1451-1457.

Lavrentyev, A.Y., A. I. Nikolaeva , N. V. Evdokimov , V. G. Semenov , V. S. Sherne, E. Y. Nemtseva , N. V. Danilova, N. S. Petrov & G. M. Toboyev. 2019. The Efficiency of The Use of “Biostrong 510” Additive in The Technology of broiler chicken meat production. IOP Conf. Ser.: Earth Environ. Sci. 346: 012053. [20 February 2020].

Lee, J. W., D. H. Kim, Y. B. Kim, S. B. Jeong, S. T. Oh, S. Y. Cho & K. W. Lee. 2020. Dietary encapsulated essential oils improve production performance of coccidiosis-vaccine-challenged broiler chickens. Animals 10:481.

Leeson, S. 2007. Balancing science versus societal issues in poultry nutrition. CAB Rev. 2:1-5.

Luis, A., S. Sousa, J. Wackerlig, D. Dobusch, A. P. Duarte, L. Pereira & F. Domingues. 2019. Star anise (Illicium verum Hook. F.) essential oil: Antioxidant properties and antibacterial activity against Acinetobacter baumannii. Flavour Frag. J. 34: 260-270.

Marcu, A., I. Vacaru-Opris, G. Dumitrescu, L. Petculescu-Ciochina, A. Marcu, M. Nicula, I. Pet, D. Dronca, B. Kelciov & C. Maris. 2013. The influence of genetics on economic efficiency of broiler chickens growth. J. Anim. Sci. Biotechnol. 46:339-346.

Mathivanan, R. & K. Kalaiarasi. 2007. Panchagavya and Andrographis panculata as alternatives to antibiotic growth promoters on hematological, serum biochemical parameters and immune status of broilers. J. Poult. Sci. 44:198-204.

Meimandipour, A., A. N. Emamzadeh, & A. Soleimani. 2017. Effects of nanoencapsulated aloevera, dill and nettle root extract as feed antibiotic substitutes in broiler chickens. Arch. Anim. Breed. 60:1-7.

Miladi, H., R. Ben Slama, D. Mili, S. Zouari, A. Bakhrouf & E. Ammar. 2013. Chemical composition and cytotoxic and antioxidant activities of Satureja montana L. essential oil and its antibacterial potential against Salmonella Spp. strains. J. Chem. 2013:1-9.

Mora, Z. V.-D., M. E. Macías-Rodríguez, J. Arratia-Quijada, Y. S. Gonzalez-Torres, K. Nuño & A. Villarruel-López. 2020. Clostridium perfringens as foodborne pathogen in broiler production: Pathophysiology and potential strategies for controlling necrotic enteritis. Animals 10:1718.

Moslehi, H., B. Navidshad, S. D. Sharifi & F. M. Aghjegheshlagh. 2019. Effects of selenium and flaxseed on selenium content and antioxidant properties of eggs and immune response in hens. S. Afr. J. Anim. Sci. 49:770-780.

Mwangi, S., J. Timmons, S. Fitz-Coy & S. Parveen. 2018. Characterization of Clostridium perfringens recovered from broiler chicken affected by necrotic enteritis. Poult. Sci. 98:128-135.

Navidshad, B., B. Darabighane & M. Malecky. 2018. Garlic: An alternative to antibiotics in poultry production, a review. Iran. J. Appl. Anim. Sci. 8:9-17.

Panel on Additives and Products or Substances Used in Animal Feed. 2016. Safety and efficacy of BIOSTRONG® 510 (essential oil of thyme and star anise) for chickens and minor avian species for fattening and rearing to point of lay. EFSA J. 14:4351.

Paradis, M. A., E. McMillan, R. Bagg, G. Vessie, A. Zocche & M. Thompson. 2016. Efficacy of avilamycin for the prevention of necrotic enteritis caused by a pathogenic strain of Clostridium perfringens in broiler chickens. Avian Pathol. 45:365-369.

Peterson, A. L., M. A. Qureshi, P. R. Ferket & J. C. Fuller Jr. 1999. Enhancement of cellular and humoral immunity in young broilers by the dietary supplementation of β-hydroxy-β-methylbutyrate. Immunopharmacol. Immunotoxicol. 21:307-330.

Sadeghi, A. A. & M. Moghaddam. 2018. The effects of turmeric, cinnamon, ginger and garlic powder nutrition on antioxidant enzymes’ status and hormones involved in energy metabolism of broilers during heat stress. Iran. J. Appl. Anim. Sci. 8:125-130.

Saleh, A. A., T. A. Ebeid & A. M. Abudabos. 2018. Effect of dietary phytogenics (herbal mixture) supplementation on growth performance, nutrient utilization, antioxidative properties, and immune response in broilers. Environ. Sci. Pollut. Res. 25:14606-14613.

SAS. 2003. User’s guide: Statistics Version 9.1. Statistical Analysis Software Institute, Cary, North Carolina.

Singh J. & D. S. Gaikwad. 2020. Phytogenic feed additives in animal nutrition. In: Singh, J. & Yadav, A. (Eds). Natural Bioactive Products in Sustainable Agriculture. Springer, Singapore. p. 273-289.

Sun, W., M. H. Shahrajabian, Q. Cheng & S. Sabatini. 2019. Anise (Pimpinella anisum L.), a dominant spice and traditional medicinal herb for both food and medicinal purposes. Cogent Biol. 5:1-25.

Teixeira, B., A. Marques, C. Ramos, C. Serrano, O. Matos, N. R. Neng, J. M. F. Nogueira, J. A. Saraiva & M. L. Nunes. 2013. Chemical composition and bioactivity of different oregano (Origanum vulgare) extracts and essential oil. J. Sci. Food Agric. 93: 2707-2714.

Vicente, J., A. Wolfenden, A. Torres-Rodriguez, S. Higgins, G. Tellez & B. Hargis. 2007. Effect of a Lactobacillus species-based probiotic and dietary lactose prebiotic on turkey poultry performance with or without Salmonella enteritidis challenge. J. Appl. Poult. Res. 16: 361-364.

Yakhkeshi, S., S. Rahimi & K. Gharib Naseri. 2011. The effects of comparison of herbal extracts, antibiotics, probiotics and organic acid on serum lipids, immune response, GIT microbial population, intestinal morphology and performance of broilers. J. Med. Plant. 10:80-95.

Yalcin, S., S. Yalcin, H. Eser, A. Sahin, S. S. Yalcin & S. Gucer. 2014. Effects of dietary yeast cell wall supplementation on performance, on carcass characteristics, antibody production and histopathological changes in broilers. Kafkas Univ. Vet. Fak. Derg. 20:757-764.

Yang, C., M. A. Chowdhury, Y. Huo & J. Gong. 2015. Phytogenic compounds as alternatives to in-feed antibiotics: Potentials and challenges in application. Pathogens. 4:137-156.

Zeng, Z., S. Zhang, H. Wang & X. Piao. 2015. Essential oil and aromatic plants as feed additives in non-ruminant nutrition: A review. J. Anim. Sci. Biotechnol. 6:1-10.

How to Cite
Masoud-Moghaddam, S., Mehrzad, J., Alizadeh-Ghamsari, A. H., Bahari Kashani, R., & Saeidi, J. (2021). Comparison of Different Herbal Additives on Immune Response and Growth Performance of Broiler Chickens. Tropical Animal Science Journal, 44(3), 327-335.