Association between GH (g.1456_1457insT), GHRH (g.4474 C>A), and Pit-1 (g.244G>A) Polymorphisms and Lactation Traits in Holstein Friesian Cattle

A. Anggraeni, C. Sumantri, F. Saputra, L. Praharani


Lactation traits are controlled by many genes, among others, potentially by growth genes. This research was conducted to study genetic polymorphisms of GH, GHRH, and Pit-1 genes and associations of GH (g.1456_1457 InsT), GHRH (g.4474 C>A), and Pit-1 (g.244 G>A) genotypes with milk yield and quality in Holstein Friesian (HF) cattle. Genotyping was conducted for HF dairy cows raised by small farmers from North Lembang (SF-NL) (98 heads) and South Lembang (SF-SL) (95 heads), and also from Cikole Dairy Cattle Station (CDCS) (82 heads) in Lembang, West Java, Indonesia. Progeny tested of HF bulls (17 heads) from LAIC (Lembang Artificial Insemination Center) in West Java and from SAIC (Singosari AIC) (32 heads) in East Java were also genotyped. Effects of genotypes on test day milk yield, fat content, and SNF content were analyzed by General Linear Models. The GHRH g.4474 C>A SNP and Pit-1 g.244 G>A SNP generated high frequencies of C allele to A allele, while the two allelic frequencies of the GH g.1456_1457 InsT varied. Compared to the AA and AC genotypes, the CC genotype of the GH gene resulted higher test day milk yield (p<0.01), fat content (p<0.05), and SNF content (p<0.05). Further the CC genotype of the GHRH gene yielded higher milk yield (p<0.05), while the GG genotype of the Pit-1 gene resulted higher fat content (p<0.05). Therefore the GH g.1456_1457 InsT, GHRH g.4474 C>A SNP, and Pit-1 g.244 G>A SNP are potential to be used as molecular markers for selection on milk yield and quality in domestic HF cattle.


Ahmadi, M. M., A. Mirzaei, H. Sharifiyazdi, A. Hajibemani, & A. G. Rowshan. 2015. Pituitary-specific transcription factor 1 (Pit-1) polymorphism and its association on milk production and some reproductive performance in Holstein dairy cows. Rev. Med. Vet. 166:127-131.

Ahmadzadeh, M., F. Rashidi, H. A. Najafabadi, A. Jaferian, & S. Eghbalsaied. 2019. Effects of genetic polymorphism in Pit1, GH, GHR and KCN3 on milk yield and body weight of Khuzestan (Iran) water buffaloes. Rev. Colomb. Cienc. Pec. 32:107-116.

Amiri, S., B. Jemmali, M. Amine Ferchichi, H. Jeljeli, R. Boulbaba, & A. Ben Gara. 2018. Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia. Arch. Anim. Breed. 61:481-489.

Anggraeni, A. 2012. Perbaikan genetik sifat produksi susu dan kualitas susu saoi Friesian Holstein melalui seleksi. Wartazoa 22:1-11.

Anggraeni, A., H. S. Nury, E. Andreas, & C. Sumantri. 2017. Genetic variants of k-Casein and β-Lactoglobulin genes and their association with protein and milk components of Holstein Friesian cows at small farmers in Lembang, West Java. The 2nd International Conference on Sustainable Agriculture and Food Security: A Comprehensive Approach, KnE Life Sciences. pp. 86–94.

Arango, J., J. J. Echeverri, & A. López. 2014. Association between a polymorphism in intron 3 of the bovine growth hormone gene and growth traits in Holstein heifers in Antioquia. Genet. Mol. Res. 13:6191-6199.

Ardicli, S., H. Samli, B. Soyudal, D. Dincel, & F. Balci. 2019. Evaluation of candidate gene effects and environmental factors on reproductive performance of Holstein cows. S. Afr. J. Anim. Sci. 49: 379-294.

Aytekin, I. & S. Boztepe. 2013. Associations of Pit-1 gene polymorphism with milk yield and composition traits in Brown Swiss cattle. J. Anim. Plant Sci. 23:1281-1289.

Bayram, D., K. Arslan, B. Akyüz, & K. M. Isçan. 2017. Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey. Ankara. Univ. Vet. Fak. Derg. 64:337-343.

Doosti, A., A. Arshi, & B. Momeni. 2011. Molecular study of PIT1 gene polymorphism in holstein and Iranian native cattle. Afr. J. Agric Res. 6: 4467-4470.

Dybus, A. 2002. Associations of growth hormone (GH) and prolactin (PRL) genes polymorphisms with milk production traits in Polish Black-and-White cattle. Anim Sci. Pap. Rep. 20: 203-212.

Dybus, A., I. Szatkowska, E. Czerniawska-Piatkowska, W. Grzesiak, J. Wójcik, E. Rzewucka, & S. Zych. 2004. PIT1-HinfI gene polymorphism and its associations with milk production traits in polish Black-and-White cattle. Arch. Anim. Breed. 47: 557-563.

Hartati, H., B. Soewandi, A. Hapsari, S. Anwar, & D. Pamungkas. 2019. Identification of GH|MspI and GHR|AluI gene polymorphism and its association with calf birth weight of Grati-PO cattle. Jurnal Ilmu Ternak dan Veteriner 24: 49-55.

Hartatik, T., D. Kurniawati, & Adiarto. 2015. Associations between polymorphism of growth hormone gene with milk production, fat and protein content in Friesian Holstein cattle. J. Indones. Trop. Anim. Agric. 40: 133-137.

Hoseinzadeh, Z. E., M. R. Mohammadabadi, A. Esmailizadeh, A. Khezri, & A. N. Noori. 2015. Association of PIT1 gene with milk fat percentage in Holstein Cattle. J. Livest. Sci. Technol. 3: 41-49.

Huai, Y. T., X. Y. Lan, L. Ma, J. Wang, C. Z. Lei, H. Chen, & J. Q. Wang. 2011. Novel mutation in TGA stop-codon of bovine SIX6 gene. Mol. Biol. 45: 218-224.

Kiyici, J. M., K. Arslan, B. Akyuz, M. Kaliber, E. G. Aksel, & M. U. Çinar. 2019. Relationships between polymorphisms of growth hormone, leptin and myogenic factor 5 genes with some milk yield traits in Holstein dairy cows. Int. J. Dairy Technol. 72: 1-7.

Ma, L., Q. Qin, Q. Yang, M. Zhang, H. Zhao, C. Pan, C. Lei, H. Chen, & X. Lan. 2017. Associations of six SNPs of POU1F1-PROP1-PITX1-SIX3 pathway genes with growth traits in two Chinese indigenous goat breeds. Ann. Anim. Sci. 17:399-411.

Özdemir, M. 2012. Determination of Pit-1/Hinf1 polymorphism in Holstein and Native Ear cattle raised as genetic resource in Turkey. J. Anim. Plant Sci. 22: 25-28.

Petrini, J., L. H. S. Iung, M. A. P. Rodriguez, M. Salvian, F. Pértille, G. A. Rovadoscki, L. D. Cassoli, L. L. Coutinho, P. F. Machado, G. R. Wiggans, & G. B. Mourão. 2016. Genetic parameters for milk fatty acids, milk yield and quality traits of a Holstein cattle population reared under tropical conditions. J. Anim. Breed. Genet. 133: 384-395.

Raschia, M. A., J. P. Nani, D. O. Maizon, M. J. Beribe, A. F. Amadio, & M. A. Poli. 2018. Single nucleotide polymorphisms in candidate genes associated with milk yield in argentinean holstein and holstein x jersey cows. J. Anim. Sci. Technol. 60: 1-10.

Rini, A. O., C. Sumantri, & A. Anggraeni. 2013. GHRH|HaeIII gene polymorphism in dairy and beef cattle at national livestock breeding centers. Med. Pet. 36:185-191.

Sambrook, J., & D. W. Russell. 2001. Molecular Cloning: A Laboratory Manual, 3rd Ed. Cold Spring Harbor Laboratory Press, New York.

Sami, A. J., M. T. Nazir, Z. Jabeen, & A. Shakoori. 2011. Gene study within the 5’ flanking regions of growth hormone gene of first exon in bos indicus. Afr. J. Biotechnol. 10: 332-336.

SAS 9.1. 2011. SQL Procedure User’s Guide. SAS Institute, Cary.

Szatkowska, I., A. Dybus, W. Grzesiak, M. Jędrzejczak, & M. Muszynska. 2009. Association between the growth hormone releasing hormone (Ghrh) gene polymorphism and milk production traits of dairy cattle. J. Appl. Anim. Res. 36: 119-123.

Yeh, F., & T. Boyle. 1997. Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belg. J. Bot. 129:157-63.


A. Anggraeni (Primary Contact)
C. Sumantri
F. Saputra
L. Praharani
Author Biography

F. Saputra, Indonesian Research Institute for Animal Production (IRIAP)

Jl. Veteran III PO. BOX 22,1 Bogor

AnggraeniA., SumantriC., SaputraF., & PraharaniL. (2020). Association between GH (g.1456_1457insT), GHRH (g.4474 C>A), and Pit-1 (g.244G&gt;A) Polymorphisms and Lactation Traits in Holstein Friesian Cattle. Tropical Animal Science Journal, 43(4), 291-299.

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