The Performance, Nutrient Digestibility, Aflatoxin B1 Residue, and Histopathological Changes of Broilers Exposed to Dietary Mycosorb

C. L. Nalle, M. A. J. Supit, A. H. Angi, N. S. Yuliani


Aflatoxin B1 (AFB1) is the most dangerous type of aflatoxin that can impair poultry productivity even in low concentrations. The harmful effects of AFB1 should be minimized. The present study was conducted to evaluate the performance, nutrient digestibility, AFB1 residue, and histopathological changes of broilers fed AFB1 diets supplemented with mycosorb. Two hundred-forty 1-day old broiler chicks were allocated randomly to 24 pens (10 birds/pen). The experimental design was a 4 x 2 factorial arrangement, with AFB1 level and yeast glucomannan (mycosorb) supplementation as the main factors.  The treatment diets were control diet; control diet + mycosorb; AFB1 diets (10.36 ppb); AFB1 diets (10.36 ppb) + mycosorb; AFB1 diets (26.97 ppb); AFB1 diets (26.97 ppb) + mycosorb; AFB1 diets (61.06 ppb); AFB1 diets (61.06 ppb) + mycosorb. The results showed that there was no interaction effect was found between the level of AFB1 and mycosorb on the performance and nutrient digestibility. The level of AFB1 did not affect dry matter digestibility coefficient but affected (p<0.05) crude fat digestibility coefficient of broilers. Except for proventriculus, level of AFB1 did not affect bird’s gut size. AFB1 residue was not detected in the broiler tissues fed all diets. Mycosorb alone ameliorated (p<0.05) feed conversion ratio (FCR) of growing birds. The birds fed 61.06 ppb AFB1 diets had darker liver than those fed similar diets added with mycosorb. The addition of mycosorb in AFB1 diets did not heal the hemorrhagic intestines of the birds. In conclusion, feeding low level of AFB1 diets added with mycosorb did not improve the bird’s performance, nutrient digestibility, or gut health. Mycosorb alone improved feed efficiency of the experimental birds.


Abbasi F., J. Liu, H. Zhang, X. Shen, & X. Luo. 2018. Effects of feeding corn naturally contaminated with aflatoxin on growth performance, apparent ileal digestibility, serum hormones levels and gene expression of Na+, K+ -ATPase in ducklings. Asian-Australas. J. Anim. Sci. 31:91-97.
Afzal, M. & S. Zahid. 2004. Effects of additional mycotoxin detoxifier in poultry feed containing different levels of aflatoxins on the performance of broilers. Asian-Aust. J. Anim.Sci. 17:990-994.
Aly, S. A. & W. Anwer. 2009. Effect of Naturally Contaminated Feed with Aflatoxins on Performance of Laying Hens and the Carryover of Aflatoxin B Residues in Table Eggs. Pakistan J. Nut. 8: 181-186.
AOAC. 2005. Official Methods of Analysis of AOAC International. 18th ed. Assoc. Off. Anal. Chem., Arlington.
Azeem, N., M. Nawaz, A. A. Anjum, S. Saeed, S. Sana, A. Mustafa, & M. R. Yousuf. 2019. Activity and Anti-Aflatoxigenic Effect of Indigenously Characterized Probiotic Lactobacilli against Aspergillus flavus-A Common Poultry Feed Contaminant. Animals. 9:166.
Barati, M., M. Chamani, S. N. Mousavi, S. A. Hoseini, M. T. A. Ebrahimi. 2017. Effects of biological and mineral compounds in aflatoxin-contaminated diets on blood parameters and immune response of broiler chickens. J. App. Anim. Res. 46:707-713.
Benkerroum, N. 2020. Chronic and acute toxicities of aflatoxins: mechanisms of action. Int. J. Environ. Res. Public Health.17:423.
Bryden, W. L. 2012. Mycotoxin Contamination of the Feed Supply Chain : Implications for Animal Productivity and Feed Security. Anim. Feed. Sci. Tech. 173:134-138.
Campos-M M. & R. Campos-C. 2017. Application of quartering method in soils and foods. Int. J. Eng. Res. App. 7:35-39.
Carvajal-Moreno A. 2015. Metabolic changes of aflatoxin B1 to become an active carcinogen and the control of this toxin. Immunome. Res. 11:2
Chu, Y., H.I Yang, Hui-Chen Wu, J. Liu, L. Wang, S. Lu, M. Lee, C. Jen, S. You, R. M. Santella, & C. Chen. 2017. Aflatoxin B1 exposure increases the risk of cirrhosis and hepatocellular carcinoma in chronic hepatitis B virus carriers. Int. J. Cancer. 141: 711-720.
Diaz, G. J. & H. W. Murcia. 2011. Biotransformation of Aflatoxin B1 and Its Relationship with The Differential Toxicological Response to Aflatoxin in Commercial Poultry Species. In Aflatoxins: Biochem. Mol. Biol. p. 3-20.
Fani Makki, O., N. Afzali, & A. Omidi. 2013. Effect of different levels of Silymarin (Silybum marianum) on growth rate, carcass variables and liver morphology of broiler chickens contaminated with aflatoxin B1. Poult. Sci. J. 1: 105-116.
Feng, G. D., J. He, X. Ao, & D. W. Chen. 2017. Effects of maize naturally contaminated with aflatoxin B1 on growth performance, intestinal morphology, and digestive physiology in ducks. Poult. Sci. 96:1948-1955.
Fouad, A. M., D. Ruan, H. K. El-Senonseuj, W. Chen, S. Jiang, & C. Zheng. 2019. Harmful effects and control strategies of aflatoxin B1 produced by Aspergillus flavus and Aspergillus parasiticus strains on poultry: review. Toxins. 11:176.
Galarza-Seeber, R., J. D. Latorre, L. R. Bielke, V. A. Kuttappan, A. D. Wolfenden, X. Hernandez-Velasco, R. Merino-Guzman, J. L. Vicente, A. Donoghue, D. Cross, B. M. Hargis, & G. Tellez. 2016. Leaky gut and mycotoxins: Aflatoxin B1 does not increase gut permeability in broiler chickens. Front. Vet. Sci. 3:10.
Garcia-Moraleja, A., G. Front, J. Manes, & E. Ferrer. 2015. Development of a new method for the simultaneous determination of 21 mycotoxins in coffee beverages by liquid chromatography tandem mass spectrometry. Food Res. Int. 72:247-255.
Girish, C. K & G. Devegowda. 2006. Efficacy of glucomannan-containing yeast product (mycosorb®) and hydrated sodium calcium aluminosilicate in preventing the individual and combined toxicity of aflatoxin and T2-toxin in commercial broilers. Asian-Aust. J. Anim. Sci. 19:877-883.
Goodarzi, E, F. Ghoratt, A. M. Jerrahi, H. A. Adinehs, M. Sohrabivafa, & Z. Khazaei. 2018. Global incidence and mortality of liver cancers and its relationship with the human development index (HDI): an ecology study in 2018. World Cancer Res. J. 6:e1255.
Grenier, B. & T. J. Applegate. 2013. Modulation of Intestinal Functions Following Mycotoxin Ingestion: Meta-Analysis of Published Experiments in Animals. Toxins. 5:396-430.
Han, X.Y., H. Qi-Chun, L. Wei-Fen, J. Jun-Fang, & X. Rong. 2008. Changes in growth performance, digestive enzyme activities and nutrient digestibility of cherry valley ducks in response to aflatoxin B1 levels. Livest. Sci. 119:216-220.
Hussain, Z., H. Rehman, S. Manzoor, S. Tahir, & M. Muchtar. 2016. Determination of liver and muscle aflatoxin B1 residues and select serum chemistry variables during chronic aflatoxicosis in broiler chickens. Vet. Clin. Pathol. 45:330-334.
Jettanajit, A. & T. Nhujak. 2016. Determination of mycotoxins in brown rice using QuEChERS sample preparation and UHPLC-MS-MS. J. Chromatogr. Sci. 54: 720-729.
Kumar, R. & C. Balachandran. 2009. Histopathological changes in broiler chickens fed aflatoxin and cyclopiazonic acid. Vet. Archiv. 79:31-40.
Kurniasih & Y. A. Prakoso. 2019. Recent update: effects of aflatoxin in broiler chickens. J. World’s Poult. Res. 9:68-77.
Latimer, G. W. 2005. AOAC International. Official methods of analysis of AOAC International. 19th ed. Gaithersburg, MD: AOAC International.
Liu, N., J. Wang, Q. Deng, K. Gu, & J. Wang. 2018. Detoxification of aflatoxin B1 by lactic acid bacteria and hydrated sodium calcium aluminosilicate in broiler chickens. Livest. Sci. 208:28-32.
Magnoli, A. P., M. P. Monge, R. D. Miazzo, L. R. Cavaglieri, C. E. Magnoli, C. I. Merkis, A. L. Cristofolini, A. M. Dalcero, & S. M. Chiacchiera. 2011. Effect of low level of aflatoxin B1 on performance, biochemical parameters and aflatoxin B1 in broiler liver tissues in the presence of monensin and sodium bentonite. Poult. Sci. 90:48-58.
Marchioro, A. A., A. O. Mallmann, A. Diel, P. Dilkin, R. H. Rauber, F. J. H Blazquez, & C. M. G. A. Oliveira. 2013. Effects of Aflatoxins on Performance and Exocrine Pancreas of Broiler Chickens Effects of Aflatoxins on Performance and Exocrine Pancreas of Broiler Chickens. American Assoc. Avian Path. 57:280-284.
Matur, E., E. Ergul, I. Akyazi, E. Eraslan, & Z. T. Cirakli. 2010. The effects of Saccharomyces cerevisiae extract on the weight of some organs, liver, and pancreatic digestive enzyme activity in breeder hens fed diets contaminated with aflatoxins. Poult. Sci. 89:2213-20.
McDonald. P., R. A. Edwards. J. F. D. Greenhalgh, & C. A. Morgan. 2002. Animal Nutrition. 6th ed. Prentice Hall, United Kingdom.
Mogadam, N. & A. Azizpour. 2011. Ameliorative effect of glucomannan-containing yeast products (Mycosorb) and sodium bentonite on performance and antibody titers against Newcastle disease in broilers during chronic aflatoxicosis. African J. Biotech. 10:17372-17378.
Moran, C. A, J. Apalajahti, A. Yiannikouris, S. Ojanpera, & H. Kettunen. 2013. Effects of low dietary aflatoxin B1 on broiler liver concentration without and with mycosorb® toxin binder. J. Appl. Anim. Nut. 2:1-3.
Nalle, C. L., V. Ravindran, & G. Ravindran. 2010. Nutritional value of faba beans (Vicia faba L.) for broilers: Apparent metabolisable energy, ileal amino acid digestibility and production performance. Anim. Feed Sci. Tech. 156:104-111.
Nalle, C. L, V. Ravindran V, & G. Ravindran. 2011. Nutritional Value of Narrow-Leafed Lupin (Lupinus angustifolius) for Broilers. Br. Poult. Sci. 2:775-81.
Nalle, C. L., A. H. Angi, M. A. J. Supit, & S Ambarwati. 2019. Aflatoxin and ochratoxin A contamination in corn grains and sago (putak meal) from different sources for poultry in West Timor, Indonesia. Int. J. Poult. Sci. 18:353-360.
Nazarizadeh, H. & J. Pourreza. 2019. Evaluation of three mycotoxin binders to prevent the adverse effects of aflatoxin B1 in growing broilers. J. Appl. Anim. Res. 47:135-139.
Ortatatli, M., H. Oguz, F. Hatipoglu, & M. Karaman. 2005. Evaluation of pathological changes in broilers during chronic aflatoxin (50 and 100 ppb) and clinoptilolite exposure. J. Res. Vet. Sci. 78:61-68.
Peng, X., S. Bai, X. Ding, & K. Zhang. 2017. Pathological impairment, cell cycle arrest and apoptosis of thymus and bursa of fabricius induced by aflatoxin-contaminated corn in Broilers. Int. J. Environ. Res. Public Health. 14:77.
Resanovic, R. & Z. Sinovec. 2006. Effects of limited feeding of AFB1 contaminated feed on the performance of broilers. Mycotoxin Res. 22:183-188.
Rotimi, O. A., S. O. Rotimi, J. M. Goodrich, I. B. Adelani, E. Agbonihale, & G. Talabi. 2019. Time-course effects of acute aflatoxin B1 exposure on hepatic mitochondrial lipids and oxidative stress in rats. Front. Pharmacol. 10:467.
Rotimi, O. A., S. O. Rotimi, C. U. Duru, O. J. Ebebeinwe, A. O. Abiodun, B. O. Oyeniyi, F. A. Faduyile. 2017. Acute aflatoxin B1-Induced hepatotoxicity alters gene expression and disrupts lipid and lipoprotein metabolism in rats. Tox. Rep. 4:408-414. 10.1016/j.toxrep.2017.07.006
Rushing, B. R. & M. I. Selim. 2018. Aflatoxin B1: a review on metabolism, toxicity, occurrence in food, occupational exposure, and detoxification methods. Food Chem. Toxicol. 124, 81-100. 10.1016/j.fct.2018.11.047
Saki, A., A. Rahmani A, H. Mahmoudi, H. M. M. Tabatabaei, P. Zamani, & A. R. Khosravi. 2018. The ameliorative effect of mycosorb in aflatoxin contaminated diet of broiler chickens. J. Livest. Sci. Tech. 6:39-47.
Solis-Cruz, B., D. Hernandez-Patlan, V. M. Petrone, K. P. Pontin, J. D. Latorre, E. Beyssac, X. Hernandez-Velasco, R. Merino-Guzman, C. Owens, B. M. Hargis, R. Lopez-Arellano, & G. Tellez-Isaias. 2019. Evaluation of cellulosic polymers and curcumin to reduce Aflatoxin B1 toxic effects on performance, biochemical, and immunological parameters of broiler chickens. Toxins. 11:121.
SAS Institute. SAS/STAT® User's Guide: Statistics. (SAS® University Edition- Free Online Software). SAS Institute Inc., Cary, NC, USA.
Sineque, A. R., C. L. Macuamule, & F. R. Dos Anjos. 2017. Aflatoxin B1 Contamination in Chicken Livers and Gizzards from Industrial and Small Abattoirs, Measured by ELISA Technique in Maputo, Mozambique. Int. J. Environ. Res. Public Health. 14:951.
Saleemi, M. K., K. Ashraf, S. T. Gula, M. N. Naseema, M. S. Sajid, M. Mohsin, C. He, M. Zubair, & A. Khan. 2020. Toxicopathological effects of feeding aflatoxins B1 in broilers and its amelioration with indigenous mycotoxin binder. Ecotox. Env. Safety. 187: 109932.
Suganthi, R. U., K. P. Suresh, & R. Parvatham. 2011. Effect of aflatoxin on feed conversion ratio in broilers. J. Anim. Sci. 24:1757-1762.
The American Cancer Society. 2019. Liver Cancer Early Detection, Diagnosis, and Staging. . [9 July 2020]
Wade, M. R., D. Sapcota, & U. Verma. 2017. Ameliorating aflatoxicosis in commercial broiler chickens by dietary Mycosorb: Haemato-Biochemical studies. Indian J. Anim. Res. B 3182: 1-5.
Wade, M. R. & D. Sapcota. 2015. Aflatoxicosis in broiler chickens, ameliorating with mycosorb : a review. Int. J. Curr. Res. 7:17487-17490.
Wu, Hui-Chen & R. Santella. 2012. The role of aflatoxins in hepatocellular carcinoma. Hepat Mon. 12:e7238.
Yang, J., F. Bai, K. Zhang, X. Lv, S. Bai, L. Zhao, X. Peng, X. Ding, Y. Li, J. Zhang. 2012. Effects of feeding corn naturally contaminated with AFB1 and AFB2 on performance and aflatoxin residue in broilers. Czech J. Anim. Sci. 57:506-515.
Yunus, A. W., E. Razzazi_Fazeli, & J. Bohm. 2011. Aflatoxin B1 affecting broiler’s performance, immunity and gastrointestinal tracts: a review of history and contemporary issues. Toxins. 3:566-590.


C. L. Nalle (Primary Contact)
M. A. J. Supit
A. H. Angi
N. S. Yuliani
Author Biographies

C. L. Nalle, Feed Technology Study Program, Department of Animal Husbandry, State Polytechnic of Agriculture Kupang

Dr. Catootjie L. Nalle is an associate professor at Feed Technology Study Program- Kupang State Agriculture Polytechnic. I finished my PhD study in Nutritional Science from Massey University, New Zealand in 2009 with thesis entitled “Nutritional evaluation of grain legumes for poultry”. I have been working as a lecturer since 1995. Subjects that I teach are 1) Feed toxicology; 2) Feed control quality; 3) Poultry feed technology; 4) Poultry nutrition; 5) Experimental design; 6) Laboratory technique; 7) Processing of non-ruminant concentrate. I have participated in a number of training such as HACCP, mycotoxin analysis for feed and food. Since 2005 up to now, I have been doing a number of research about grain legumes, sago, mycotoxin, enzyme, and symbiotic in broiler ration. I have published my research articles in Animal Feed Science and Technology Journal (Elsevier), British Poultry Science, Animal, Animal Production Science, American Journal of Animal and Veterinary Science, Journal of Science and Food Agriculture, International Journal of Poultry Science, Pakistan Journal Nutrition, and International Journal of Agriculture System. (E-mail address:;;)

M. A. J. Supit, Animal Production Study Program, Department of Animal Husbandry, State Polytechnic of Agriculture Kupang

Max A.J. Supit, M.Food.Tech is a senior lecturer at Animal Production Study Program Husbandry Department of Kupang State Agricultural Polytechnic. I finished my Master Degree in Food Science and Technology, University of Newcastle, NSE, Australia in 2006 with a thesis entitled “Effect of  Kesambi (Schleichera oleosa) Liquid Smoke Application on Textural Properties of Beef”. I have been working as lecturer since 2004. Subjects that I teach are 1). Experimental Design 2) Statistics 3) Animal Food Production Technology 4) Feed Quality Control. I have conducted and also involved in a number of research about the use of liquid smoke on smoked beef and pork and on production of ready to use smoked seasoning as well as mycotoxin in feed which all related to food/feed safety. As member of research team I have published research articles in International Journal of Poultry Science and Pakistan Journal Nutrition.  . (E-mail address:;

A. H. Angi, Animal Health Study Program, Department of Animal Husbandry, State Polytechnic of Agriculture Kupang


Dr. Andrijanto H. Angi is an associate professor at Animal Health Study Program-Kupang State Agriculture Polytechnic. I finished my Doctoral degree in Veterinary Science from Bogor Agriculture University, Indonesia in 2015 with dissertation entitled “Prevalence of trichinellosis in pigs in Kupang city slaughterhouse of East Nusa Tenggara Province”. I have been working as a lecturer since 2002. Subjects that I teach are 1) Feed toxicology; 2) Feed control quality; 3) Bacterial and Fungy; 4) Microbiology; 5) Animal Health. I have been doing a number of research about sago, mycotoxin, feed enzymes and symbiotic in broiler ration. I have published my research articles Global Veterinaria Journal, Veterinary Journal, Veterinary Investigate Journal. (E-mail address:;;)

N. S. Yuliani, Animal Health Study Program, Department of Animal Husbandry, State Polytechnic of Agriculture Kupang

Ni Sri Yuliani, M.Vet.Sc. is a senior lecturer at Animal Health Study Program-Kupang State Agriculture Polytechnic. I finished my Master degree in Pathology at Veterinary Science Study Program, University of Gadjah Mada, Indonesia in 2012 with thesis entitled “The efficacy of ethanol extract of Chromolaena odorata on the wound healing of Sprague Dawley mouse”. I have been working as a lecturer since 2008. Subjects that I teach are 1) Pathology; 2) Anatomy; 3) Bacterial and Fungal Disease and 4) Veterinary community health. I have been doing a number of research about phytochemical compound of Indonesian spices and plants. I have published my research articles in some national journals. (E-mail address:;;)

NalleC. L., SupitM. A. J., AngiA. H., & YulianiN. S. (2021). The Performance, Nutrient Digestibility, Aflatoxin B1 Residue, and Histopathological Changes of Broilers Exposed to Dietary Mycosorb. Tropical Animal Science Journal, 44(2), 160-172.

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