Immune Status and Growth of Broiler Fed Diet with Microparticle Protein Added with Natural Acidifier

N. Suthama, B. Sukamto, I. Mangisah, L. Krismiyanto


The present study was conducted to evaluate the feeding effect of diet composed of microparticle protein added with lime juice as an acidifier on immune status and efficiency of protein utilization in relation to the growth of broiler chicken. Dietary treatments tested were P1 was diet with 20% intact protein and CaCO3 as a Ca source, P2 was diet with 20% microparticle protein and eggshell as a Ca source added with 1.2% lime juice, and P3 was diet with 20% microparticle protein and eggshell as a Ca source added with 2.4% lime juice. Variables measured were protein and amino acids digestibility, retentions of N and Ca, fecal Nτ–methyl histidine (Nτ–MH) excretion as a protein turnover index, lymphoid organs, heterophil and lymphocyte (H/L) ratio, immunoglobulin A (IgA) as a body resistance indicator, feed consumption, body weight gain (BWG), and feed conversion ratio (FCR). The experiment was arranged in a completely randomized design with 3 treatments and 8 replications. Data were subjected to analysis of variance and Duncan test at 5% probability. Protein and amino acid digestibility, N, and Ca retentions were significantly (p<0.05) higher in P2 and P3. However, the H/L ratio was significantly (p<0.05) lower in both treatments compared to P1. Bursa fabricius and lymph weights were not difference, but BWG in P2 significantly (p<0.05) increased. In conclusion, protein and some essential amino acids digestibility, N and Ca retentions, IgA, and BWG increase with the decrease in H/L ratio, fecal Nτ–MH, feed consumption, and FCR in broiler fed diet composed of microparticle protein source with additional 1.2 mL acidifier of lime juice.


Abdelrazek, H. M. A., S. M. M. Abuzead, S. A. Ali, H. M. A. El-Genaidy, & S. A. Abdel-Hafez. 2016. Effect of citric and acetic acid water acidification on broiler’s performance with respect to thyroid hormones levels. Adv. Anim. Vet. Sci. 4:271-278.
Abdurrahman, Z. H., Y. B. Pramono, & N. Suthama. 2016a. Feeding effect of inulin derived from dahlia tuber combined with Lactobacillus sp. on meat protein mass of crossbred kampong chicken. J. Indones. Trop. Anim. Agric. 41:37-44.
Abdurrahman, Z. H., Y. B. Pramono, & N. Suthama. 2016b. Meat characteristic of crossbred local chicken fed inulin of dahlia tuber and Lactobacillus sp. Med. Pet. 39:112-118.
Adil, S., T. Banday, G. A. Bhat, M. S. Mir, & M. Rehman. 2010. Effect of dietary supplementation of organic acids on performance, intestinal histomorphology, and serum biochemistry of broiler chicken. Vet. Med. Int. 20:1-7.
Al-Jaf, K. A. H. & Y. K. Del. 2019. Effect of different feed additives on growth performance and production in livestock. Int. J. Agric. For. 9:16-31.
Amerah, A. M., V. Ravindran, R. G. Lentle, & D. G. Thomas. 2008. Influence of feed particle size on the performance, energy utilization, digestive tract development, and digesta parameters of broiler fed wheat- and corn-based diets. Poult. Sci. 87:2320-2328.
Castanon, J. I. R. 2007. History of the use of antibiotic as growth promoters in European poultry feeds. Poult. Sci. 86:2466-2471.
Cholis, M.A., N. Suthama, & B. Sukamto. 2018. Feeding microparticle protein diet combined with Lactobacillus sp. on existence of intestinal bacteria and growth of broiler chickens. J. Indones. Trop. Anim. Agric. 43:265-271.
Dhama, K., R. Tiwari, R. U. Khan, S. Chakraborty, M. Gopi, K. Khartik, M. Saminathan, P. A. Desingu, & L. T. Sunkara. 2014. Growth promoter and novel feed additives improving poultry production and health, bioactives principles and beneficial applications: The trends and advances - A review. Int. J. Pharmacol. 13:129-159.
Driver, J. P., G. M. Pesti, R. I. Bakalli, & H. M. Edwards, Jr. 2005. Calcium requirements of the modern broiler chicken as influenced by dietary protein and age. Poult. Sci. 84:1629-1639.
Fajrih, N., N. Suthama, & V. D. Yunianto. 2014. Body resistance and productive performances of crossbred local chicken fed inulin of dahlia tubers. Med. Pet. 37: 108-114.
Fleet, J. M. & R. D. Schoch. 2010. Molecular mechanisms for regulation of intestinal Calcium absorption by vitamin D and other factors. Crit. Rev. Clin. Lab. Sci. 47:181-195.
Goh, C. H., T. C. Loh, H. L. Foo, & F. Nobilly. 2020. Fecal microbial population and growth in broiler fed organic acids and palm fat-composed diet. Trop. Anim. Sci. J. 43:151-157.
Hayajneh, F. M. F. 2019. Natural feed additives for broiler chickens. S. Afr. J. Anim. Sci. 49:867-873.
Huang, C. & H. H. Stein. 2016. Amino acid digestibility in soy protein concentrate with different particle sizes fed to weanling pigs. Pig Progress Res. Report. p. 32-33.
Jambrak, A. R., T. J. Mason, V. Lelas, L. Paniwnyk, & Z. Herceg. 2014. Effect of ultrasound treatment on particle size and molecular weight of whey proteins. J. Food Eng. 121:15-23.
Jamilah, N. Suthama, & L. D. Mahfudz. 2013. Performa produksi dan ketahanan tubuh broiler yang diberi pakan step down dengan penambahan asam sitrat sebagai acidifier. Jurnal Ilmu Ternak dan Veteriner. 18:251-257. (In Indonesian language with English abstract).
Krismiyanto, L., N. Suthama, & H. I. Wahyuni. 2014. Feeding effect of inulin derived from Dahlia variabilis tuber on intestinal microbes in starter period of crossbred native chickens. J. Indones. Trop. Anim. Agric. 39:217-223.
Landers, T. F., B. Cohen, T. E. Wittum, & E. L. Larson. 2012. A review of antibiotic use in food animals: Perspective, policy, and potential. Public Health Rep. 127:4- 22.
Mangisah, I., B. Sukamto, H. I. Wahyuni, I. Estiningdriati, W. Saputro, & L. Krismiyanto. 2016. Nutrient digestibility and performance of male pelung chicken fed rice based ration supplemented with lime (Citrus aurantifolia) juice. Med. Pet. 39:119-124.
Mehdi, Y., M.-P. Letourneau-Montminy, M.-L. Gaucher, Y. Chorfi, G. Suresh, T. Rouissi, S. K. Brar, C. Cot , A. A. Ramirez, & S. Godbout. 2018. Use of antibiotics in broiler production: Global impacts and alternatives. Anim. Nutr. 4:170-178.
Merino-Guzman, R. , J. D. Latorre, R. Delgado, X. Hernandez-Velasco, A. D. Wolfenden, K. D. Teague, L. E. Graham, B. D. Mahaffey, M. F. A. Baxter, B. M. Hargis, & G. Tellez. 2016. Comparison of total immunoglobulin A levels in different samples in Leghorn and broiler chickens. Asian Pac. J. Trop. Biomed. 7:116-120.
Ndelekwute, E. K. & G. E. Enyenihi. 2017. Lime juice as a source of organic acids for growth and apparent nutrient digestibility of broiler chickens. J. Vet. Med. Surgery 1:1-5.
Ndelekwute, E. K., E. D. Assam, & E. M. Assam. 2018. Apparent nutrient digestibility, gut pH and digesta viscosity of broiler chickens fed acidified water. MOJ Anat. Physiol. 5:250-253.
Nourmohammadi, R. & N. Afzali. 2013. Effect of citric acid and microbial phytase on small intestinal morphology in broiler chicken. Ital. J. Anim. Sci. 12:44-47.
Pacheco, W. J., C. R. Stark, P. R. Ferket, & J. Brake. 2013. Evaluation of soybean meal source and particle size on broiler performance, nutrient digestibility, and gizzard development. Poult. Sci. 92:2914-2922.
Pan, D. & Z. Yu. 2014. Intestinal microbiome of poultry and its interaction with host and diet. Gut Microbes. 5:108-119.
Perdinan, A., H. I. Wahyuni, & N. Suthama. 2019. Body resistance and growth performance of broiler fed glucomannan extracted from Amorphophallus onchophyllus Tuber. Trop. Anim. Sci. J. 42:33-38.
Purbarani, S. A., H. I. Wahyuni, & N. Suthama. 2019. Dahlia Inulin and Lactobacillus sp. in step down protein diet on villi development and growth of KUB chickens. Trop. Anim. Sci. J. 42:19-24.
Ravindran, V., L. I. Hew, G. Ravindran, & W. L. Bryden. 1999. A comparison of ileal digesta and excreta analysis for the determination of amino acid digestibility in food ingredients for poultry. Br. Poult. Sci. 40:266-274.
Ren, L O., F. Zhao, H. Z. Tan, J. T. Zhao, J. Z. Zhang, & H. F. Zhang. 2012. Effects of dietary protein source on the digestive enzyme activities and electrolyte composition in the small intestinal fluid of chickens. Poult. Sci. 91:1641-1646.
Risdianto, D., N. Suthama, E. Suprijatna, & S. Sunarso. 2019. Inclusion effect of ginger and turmeric mixture combined with Lactobacillus spp. isolated from rumen fluid of cattle on health status and growth of broiler. J. Indones. Trop. Anim. Agric. 44:423-433.
Rubio, A. A., J. B. Hess, W. D. Berry, W. A. Dozier III, & W. J. Pacheco. 2020. Effets of corn particle size on broiler performance during the starter, grower, and finisher periods. J. Appl. Poult. Res. 29:352-361.
Salim, H. M. D., K. S. Huque, K. M. Kamaruddin, & M. D. A. H. Beg. 2018. Global restriction of using antibiotic growth promoters and alternative strategies in poultry production. Sci. Prog. 101:52-75.
Saputra, Y. A., N. Suthama, & B. Sukamto. 2020. Feeding diets composed of low level microparticle protein derived from fish and soybean meals and using organic calcium added with Lactobacillus acidophilus or citric acid on intestinal condition and performance of broilers. Livest. Res. Rural. Dev. 32:37-53.
Sibbald, I. R. & S. Wolynetz. 1985. Estimates of retained nitrogen used to correct estimates of bioavailable energy. Poult. Sci. 64:1506-1513.
Sivaraman, S. & V. Parady. 2018. Antibiotic Use in Food Animals: Indonesia Overview. ReAct Asia-Pacific Christian Medical College, Vellore, Tamil Nadu. Section C, p. 28.
Steel, R. G. D. & J. H. Torrie. 1991. Principles and Procedures of Statistics: A Biometrical Approach. McGraw‐Hill Book Company, New York, Toronto, London
Suthama, N. 2006. Kajian aspek “protein turnover” tubuh pada ayam Kedu periode pertumbuhan. Med. Pet. 29:47-53. (In Indonesian language with English abstract).
Suthama, N. & P.J. Wibawa. 2018. Amino acids digestibility of pelleted micro-particle protein of fish meal and soybean meal in broiler chickens. J. Indones. Trop. Anim. Agric. 43:169-176.
Weimer, S. L, R. F. Wideman, C. G. Scanes, A. Mauromoustakos, K. D. Christensen, & Y. Vizzier-Thaxton. 2018. An evaluation of methods for measuring stress in broiler chickens. Poult. Sci. 97:3381-3389.
Wilkinson, S. J., P. H. Selle, M. R. Bedford, & A. J. Cowieson. 2013. Separate feeding of calcium improves performance and ileal nutrient digestibility in broiler chicks. Anim. Prod. Sci. CSIRO. Pub. p. A - G.
Yang, Y., P.A. Iji, & M. Choct. 2009. Dietary modulation of gut microflora in broiler chickens: A review of the role of six kinds of alternatives to in-feed antibiotics. World’s Poult. Sci. J. 65:97-114.
Yuanita, I., D. Sunarti, H. I. Wahyuni, & N. Suthama. 2019. Feeding Dayak onion (Eleutherine palmifolia) extract and Lactobacillus acidophilus mixture on blood biochemicals, meat quality characteristics and growth performance in broiler chickens. Livest. Res. Rural. Dev. 31:16-22.
Ziaei, N., H. Kermanshahi, & M. PileVar. 2011. Effects of dietary crude protein and calcium/phosphorus content on growth, nitrogen and mineral retention in broiler chickens. Afr. J. Biotechnol. 10:13342-13350.


N. Suthama (Primary Contact)
B. Sukamto
I. Mangisah
L. Krismiyanto
Author Biography

N. Suthama, Faculty of Animal and Agricultural Sciences, Diponegoro University Semarang

Laboratory of Nutrition and Feed Science, Department of Animal Science

SuthamaN., SukamtoB., MangisahI., & KrismiyantoL. (2021). Immune Status and Growth of Broiler Fed Diet with Microparticle Protein Added with Natural Acidifier. Tropical Animal Science Journal, 44(2), 198-204.

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