The Polymorphism and Expression of CYP2E1 Gene and Its Relation to Carcass and Meat Quality of Indonesian Lamb

R. S. Harahap, R. R. Noor, A. Gunawan


The aim of this study was to conduct the genotyping of the single nucleotide polymorphism (SNP) in g.50657948 T>G of the CYP2E1 gene and its relation to the carcass and meat quality. A total of 200 Indonesian lambs consisted of 20 Javanese fat-tail sheep (JFTS), 37 Javanese thin-tail sheep (JTTS), 20 Garut sheep (GS), 21 Jonggol sheep (JS), 34 Garut composite sheep (GCS), 35 compass agrinac sheep (CAS), and 33 Barbados cross sheep (BCS) aged between 10-12 months old were used in this study. The polymorphism of the CYP2E1 gene was characterized using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with NlaIII enzyme restriction. The CYP2E1 gene found the three genotypes (GG, GT, and TT) and the allele frequencies were in Hardy Weinberg equilibrium. The CYP2E1 gene was significantly associated with meat quality, i.e., pH value and tenderness, as well as fatty acid composition (p<0.05), i.e., saturated fatty acid (SFA): caprylic acid (C8:0), arachidic acid (C20:0), heneicosylic acid (C21:0), behenic acid (C:22:0), and tricosylic acid (C23:0), monounsaturated fatty acid (MUFA): elaidic acid (C18:1n9t) and paullinic acid (C20:1); polyunsaturated fatty acid (PUFA): linoleic acid (C18:2n6c) and y-linolenic acid (C20:3n6). A gene expression analysis indicated that the GG genotype had the highest expression level. It could be concluded that the SNP g.50657948 T>G of the CYP2E1 gene could be used for candidate marker-assisted selection to improve the carcass and meat quality of Indonesian lamb.


AOAC. 2005. Official Methods of Analysis of AOAC International. 18th ed. Assoc. Off. Anal. Chem., Arlington.
Auger, C., A. Said, P. N. Nguyen, P. Chabert, N. Idris-khodja, & V. B. Schini-kerth. 2016. Potential of food and natural products to promote endothelial and vascular health. J. Cardiovasc. Pharmacol. 68: 11-18.
Blair, H. A. & S. Dhillon. 2014. Omega-3 carboxylic acids (epanova): A review of its use in patients with severe hypertriglyceridemia. Am. J. Cardiovasc. Drugs. 14: 393-400.
Crandell, J. R., C. Tartaglia, & J. Tartaglia. 2016. Lipid effects of switching from prescription epa+dha (omega-3-acid ethyl esters) to prescription epa-only (icosapent ethyl) in dyslipidemic patients. Postgrad. Med. 128: 859-864.
Chiuve, S. E., E. B. Rimm, K. R. Sandhu, A. M. Bernstein, K. N. Rexrode, J. E. Manson, W. C. Willett, & C. M. Albert. 2012. Dietary fat quality and risk of sudden cardiac death in women. Am. J. Clin. Nutr. 96: 498-507.
Dagong, M. I. A., R. Herman, C. Sumantri, R. R. Noor, & M. Yamin. 2012. Karakteristik karkas dan sifat fisik daging domba ekor tipis (det) berdasarkan variasi genotip gen kalpastatin (cast) (lokus intron 5 - ekson 6). JITV. 17: 13-24.
Frank, D., S. T. Joo, & R. D. Warner. 2016. Consumer Acceptability of Intramuscular Fat. Korean. J. Food. Sci. Technol. 36: 699-708.
Gallo, S. B., M. B. Arrigoni, A. L. S. Lemos, M. M. H. Haguiwara, & H. V. A. Bezerra. 2019. Influence of lamb finishing system on animal performance and meat quality. Acta. Sci. Anim. Sci. 41: e44742.
Gunawan, A., R. S. Harahap, K. Listyarini, & C. Sumantri. 2019. Identification polymorphism of DGAT1 gene and its association with characteristic of carcass and fat traits in sheep. JITRO. 6: 259-266.
Gunawan, A., D. Anggrela, K. Listyarini, M. A. Abuzahra, Jakaria, M. Yamin, I. Inounu, & C. Sumantri. 2018. Identification of single nucleotide polymorphism and pathway analysis of Apolipoprotein A5 (APOA5) related to fatty acid traits in Indonesian sheep. Trop. Anim. Sci. J. 41: 165-173.
Gunawan, A., S. Sahadevan, M. U. Cinar, C. Neuhoff, C. GroßeBrinkhaus, L. Frieden, D. Tesfaye, E. Tholen, C. Looft, D. S. Wondim, & M. Holker. 2013a. Identification of the novel candidate genes and variants in boar liver tissues with divergent skatole levels using RNA deep sequencing. PLoS One. 8: p.e72298.
Gunawan, A., S. Sahadevan, C. Neuhooff, C. G. Brinkhaus, A. Gad, L. Frieden, D. Tesfaye, E. Tholen, C. Looft, M. J. Uddin, K. Schellander, & M. U. Cinar. 2013b. RNA deep sequencing reveals novel candidate genes and polymorphism in boar testis and liver tissues with divergent androstenone levels. PloS One. 8: e63259.
Harahap, R. S., A. Gunawan, & R. R. Noor. 2020. Effect of CYP2E1 gene polymorphisms on lamb odor and flavour in Indonesian sheep. The 3th International Conference of Animal Science and Technology (ICAST) 2020; 2020 Nov 3; Makassar, Indonesia.
Harris, W. S., D. Mozaffarian, E. Rimm, P. Kris-Etherton, L. L. Rudel, L. J. Appel, M. M. Engler, M. B. Engler, & F. Sacks. 2009. Omega-6 fatty acids and risk for cardiovascular disease: A science advisory from the American Heart Association Nutrition Subcommittee of the Council on Nutrition, Physical Activity, and Metabolism; Council on Cardiovascular Nursing; and Council on Epidemiology and Prevention. Circulation. 119: 902-907.
Hartl, D. L. & A. G. Clark. 1997. Principle of Population Genetic. Sunderland. Sinauer Associates.
Hocquette, J. F., F. Gondret, E. Baeza, F. Medale, C. Jurie, & D. W. Pethick. 2010. Intramuscular fat content in meat-producing animals: development, genetic and nutritional control, and identification of putative markers. Anim. 4: 303-19.
Konstandi, M., J. Cheng, & F. J. Gonzalez. 2013. Sex steroid hormones regulate constitutive expression of CYP2E1 in female mouse liver. Am. J. Physiol. Endrocrinol. Metab. 304: E1118-E1128.
Koohmaraie, M. & G. H. Geesink. 2006. Contribution of postmortem muscle biochemistry to the delivery of consistent meat quality with particular focus on the calpain system. Meat Sci. 74: 34-43.
Kubesova, A., K. Stastny, M. Faldyna, Z. Sladek, I. Steinhauserova, G. Borilova, & A. Knoll. 2019. mRNA Expression of CYP2E1, CYP2A19, CYP1A2, HSD3B, SULT1A1 and SULT2A1 genes in surgically castrated, immunologically castrated, entire male and female pigs and correlation with androstenone, skatole, indole and Improvac-specific antibody levels. CJAS. 64: 89-97.
Leung, T., R. Rajendran, S. Singh, R. Garva, M. K. Demonacos, & C. Demonacos. 2013. Cytochrome P450 2E1 (CYP2E1) regulates the response to oxidative stress and migration of breast cancer cells. BCR. 15: R107.
Li, Q., J. Chen, X. Yu, & J. M. Gao. 2019. A mini review of nervonic acid: Source, production, and biological functions. Food Chem. 301: 125286.
Lin, Z., Y. Lou, & E. J. Squires. 2006. Functional polymorphism in porcine CYP2E1 gene: Its association with skatole levels. J. Steroid. Biochem. Mol. Biol. 99: 231-237.
Listyarini, K., Jakaria, M. J. Uddin, C. Sumantri, & A. Gunawan. 2018. Association and expression of CYP2A6 AND KIF12 genes related to lamb flavour and odor. Trop. Anim. Sci. J. 41: 100-107.
Lu, Y., O. A. Young, & J. D. Brooks. 2014. Physicochemical and sensory characteristics of fermented sheep meat sausage. Food. Sci. Nutr. 2: 669-675.
Ma, X., L. Wang, Z. Shi, W. Chen, X. Yang, Y. Hu, C. Zheng, & Z. Jiang. 2019. Mechanism of continuous high temperature affecting growth performance, meat quality, and muscle biochemical properties of finishing pigs. Genes Nutr. 14: 23.
Merino, J., A. Sala-Vila, R. Kones, R. Ferre, N. Plana, J. Girona, D. Ibarretxe, M. Heras, E. Ros, & L. Masa. 2014. Increasing long-chain n-3PUFA consumption improves small peripheral artery function in patients at intermediate-high cardiovascular risk. J. Nutr. Biochem. 25: 642-646.
Mori, T. A. 2014. Omega-3 fatty acids and cardiovascular disease: epidemiology and effects on cardiometabolic risk factors. Food Funct. 5: 2004-2019.
Morlein, D., M. Lungershausen, K. Steinke, A. R. Sharifi, & C. Knorr. 2012. A single nucleotide polymorphism in the CYP2E1 gen promoter affects skatole content in backfat of boars of two commercial Duroc-sired crossbred populations. Meat Sci. 92: 739-744.
Munyaneza, J. P., A. Gunawan, & R. R. Noor. 2019. Exploring effects of betaine-homocysteine methyltransferase(BHMT) gene polymorphisms on fatty acid traits and cholesterol in sheep. JITAA. 44: 243-251.
Nei, M. & S. Kumar. 2000. Moleculear Evolution and Phylogenetics. Oxford University. New York.
Neuhoff, C., A. Gunawan, M. O. Farooq, M. U. Cinar, C. G. Brinkhaus, S. Sahadevan, L. Frieden, D. Tesfaye, E. Tholen, C. Looft, K. Schellander, & M. J. Uddin. 2015. Preliminary study of FMO1, FMO5, CYP21, ESR1, PLIN2, and SULT2A1 as candidate gene for compounds related to boar taint. Meat Sci. 108: 67-73.
Ooi, E. M. M., G. F. Watts, T. W. K. Ng, P. Hugh, & R. Barrett. 2015. Effect of dietary fatty acids on human lipoprotein metabolism: A comprehensive update. Nutrients. 7: 4416-4425.
Rasmussen, M. K., G. Zamaratskaia, & B. Ekstrand. 2011. Gender-related differences in cytochrome P450 in porcine liver-implication for activity, expression and inhibition by testicular steroids. Reprod Domes Anim. 46: 616-623.
Robic, A., C. Larzul, & M. Bonneau. 2008. Genetic and metabolic aspects of androstenone and skatole deposition in pig adipose tissue: A review. GSE. 40: 129-143.
Santos, T. C., R. S. Gates, C. F. Souza, I. F. F. Tinoco, M. G. L. Candido, & L. C. S. R. Freitas. 2019. Meat quality parameters and the effects of stress: a review. JAST-B. 9: 305-315.
Shija, D. S., L. A. Mtenga, A. E. Kimambo, G. H. Laswai, D. E. Mushi, D. M. Mgheni, A. J. Mwilawa, E. J. M. Shirima, & J. G. Safari. 2013. Chemical composition and meat quality attributes of Indigenous sheep and goats from Traditional production system in Tanzania. Asian-Aust. J. Anim. Sci. 26-295-302.
Silver, N., S. Best, J. Jiang, & S. L. Thein. 2006. Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol Biol. 7: 33.
Stupin, A., L. Rasic, A. Matic, M. Stupin, Z. Kralik, G. Kralik, M. Grcevic, & I. Drenjancevic. 2018. Omega-3 polyunsaturated fatty acids-enriched hen eggs consumption enhances microvascular reactivity in young healthy individuals. Appl. Physiol. Nutr. Metab. 43: 988-995.
Weir, N., W. Guan, B. Steffen, L. M. Steffen, A. B. Karger, & M. Y. Tsai. 2018. Omega-6 eicosadienoic Acid is associated with lower HOMA-IR and incident T2D in participants from the MESA cohort. Diabetes 67: 1560-P.
Wiercinska, P., Y. Lou, & E. J. Squires. 2012. The roles of different porcine cytochrome P450 enzymes and cytochrome b5A in skatole metabolism. Animals 6: 834-845.
Zadinova, K., R. Stupka, A. Stratil, J. Cítek, K. Vehovsky, & D. Urbanova. 2016. Boar taint - The effect of selected candidate genes associated with androstenone and skatole levels - A review. Anim. Sci. Pap. Rep. 34: 107-128.
Zadinova, K., R. Stupka, A. Stratil, J. Citek, K. Vehovsky, N. Lebedova, M. Sprysl, & M. Okrouhla. 2017. Association analysis of SNPs in the porcine CYP2E1 gen with skatole, indole, and androstenone levels in back fat of a crossbred pig population. Meat Sci. 131: 68-73.
Zimerman, M., G. Grigioni, H. Taddeo, & E. Domingo. 2011. Physiological stress responses and meat quality traits of kids subjected to different pre-slaughter stressors. Small. Rum. Res. 100: 137-142.


R. S. Harahap
R. R. Noor
A. Gunawan (Primary Contact)
HarahapR. S., NoorR. R., & GunawanA. (2021). The Polymorphism and Expression of CYP2E1 Gene and Its Relation to Carcass and Meat Quality of Indonesian Lamb. Tropical Animal Science Journal, 44(4), 377-385.

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